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pfge profiles  (New England Biolabs)


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    Structured Review

    New England Biolabs pfge profiles
    Pfge Profiles, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 808 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfge+profiles/Lambda+PFG+Ladder/10__14202_slash_vetworld__2025__270___279-104-16-22
    Average 95 stars, based on 808 article reviews
    pfge profiles - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Comparison:

    Article Title: Molecular characterization and antimicrobial-resistance gene profile of Staphylococcus aureus strains isolated from ovine mastitis in Jordan
    Article Snippet: After electrophoresis was completed, a linear ramping factor of 120u and 5–50 pulses were applied at 14°C and 6 V/cm using an electrophoresis device (Bio-Rad). .. A standard pattern was included in the gels to facilitate the comparison of the digitally normalized PFGE profiles (Lambda Ladder PFG Marker, New England Biolabs). .. Computer software (PyElph 1.4, Informer Technologies, Inc., USA) was used to obtain and analyze images of the gels.

    Article Title: Molecular characterization and antimicrobial-resistance gene profile of Staphylococcus aureus strains isolated from ovine mastitis in Jordan
    Article Snippet: After electrophoresis was completed, a linear ramping factor of 120u and 5–50 pulses were applied at 14°C and 6 V/cm using an electrophoresis device (Bio-Rad). .. A standard pattern was included in the gels to facilitate the comparison of the digitally normalized PFGE profiles (Lambda Ladder PFG Marker, New England Biolabs). .. Computer software (PyElph 1.4, Informer Technologies, Inc., USA) was used to obtain and analyze images of the gels.

    Article Title: Genetic relatedness, antibiogram and virulence factors of Staphylococcus aureus isolated from bovine mastitis and related human contacts.
    Article Snippet: The electrophoresis was then carried out using electrophoresis device (Bio-Rad, USA) for 23 hours at 14°C at 6V/cm with 5 to 50 second pulses with an angel of 120u and a linear ramping factor. .. A standard pattern was included in the gels to allow comparison of the digitally normalized PFGE profiles (Lambda Ladder PFG Marker, New England Biolabs, USA). .. Obtained gels were stained and images (containing 84 bacterial isolates patterns) were obtained and analyzed using computer software (PyElph 1.4, Informer Technologies, Inc., USA).

    Marker:

    Article Title: Molecular characterization and antimicrobial-resistance gene profile of Staphylococcus aureus strains isolated from ovine mastitis in Jordan
    Article Snippet: After electrophoresis was completed, a linear ramping factor of 120u and 5–50 pulses were applied at 14°C and 6 V/cm using an electrophoresis device (Bio-Rad). .. A standard pattern was included in the gels to facilitate the comparison of the digitally normalized PFGE profiles (Lambda Ladder PFG Marker, New England Biolabs). .. Computer software (PyElph 1.4, Informer Technologies, Inc., USA) was used to obtain and analyze images of the gels.

    Article Title: Molecular characterization and antimicrobial-resistance gene profile of Staphylococcus aureus strains isolated from ovine mastitis in Jordan
    Article Snippet: After electrophoresis was completed, a linear ramping factor of 120u and 5–50 pulses were applied at 14°C and 6 V/cm using an electrophoresis device (Bio-Rad). .. A standard pattern was included in the gels to facilitate the comparison of the digitally normalized PFGE profiles (Lambda Ladder PFG Marker, New England Biolabs). .. Computer software (PyElph 1.4, Informer Technologies, Inc., USA) was used to obtain and analyze images of the gels.

    Article Title: Genetic relatedness, antibiogram and virulence factors of Staphylococcus aureus isolated from bovine mastitis and related human contacts.
    Article Snippet: The electrophoresis was then carried out using electrophoresis device (Bio-Rad, USA) for 23 hours at 14°C at 6V/cm with 5 to 50 second pulses with an angel of 120u and a linear ramping factor. .. A standard pattern was included in the gels to allow comparison of the digitally normalized PFGE profiles (Lambda Ladder PFG Marker, New England Biolabs, USA). .. Obtained gels were stained and images (containing 84 bacterial isolates patterns) were obtained and analyzed using computer software (PyElph 1.4, Informer Technologies, Inc., USA).

    Control:

    Article Title: Similarity and Divergence of Phylogenies, Antimicrobial Susceptibilities, and Virulence Factor Profiles of Escherichia coli Isolates Causing Recurrent Urinary Tract Infections That Persist or Result from Reinfection
    Article Snippet: .. DNA fingerprints were obtained from the PFGE profiles of genomic DNA digested with XbaI (New England BioLabs) according to the procedures developed by the U.S. Centers for Disease Control and Prevention PulseNet program ( 33 ). ..

    Article Title: Characterization of KPC-2-producing Escherichia coli, Citrobacter freundii, Enterobacter cloacae, Enterobacter aerogenes, and Klebsiella oxytoca isolates from a Chinese Hospital.
    Article Snippet: Twelve nonduplicated KPC-2-producing enterobacterial isolates, including three Escherichia coli, two Citrobacter freundii, two Enterobacter cloacae, four Enterobacter aerogenes, and one Klebsiella oxytoca, were collected from various clinical samples within 18 months (March 2011 to September 2012).. Two of the 12 patients died from infections caused by KPC-2-producing pathogens, while the rest of the patients with KPC-2-producing pathogens improved or were cured.. The majority of the clinical isolates exhibited a high-level of resistance to oxyiminocephalosporins and carbapenems, and possessed self-transferable blaKPC-2-carrying plasmids with sizes ranging from 20 to 120 kb.

    Nucleic Acid Electrophoresis:

    Article Title: Characterization of KPC-2-producing Escherichia coli, Citrobacter freundii, Enterobacter cloacae, Enterobacter aerogenes, and Klebsiella oxytoca isolates from a Chinese Hospital.
    Article Snippet: Twelve nonduplicated KPC-2-producing enterobacterial isolates, including three Escherichia coli, two Citrobacter freundii, two Enterobacter cloacae, four Enterobacter aerogenes, and one Klebsiella oxytoca, were collected from various clinical samples within 18 months (March 2011 to September 2012).. Two of the 12 patients died from infections caused by KPC-2-producing pathogens, while the rest of the patients with KPC-2-producing pathogens improved or were cured.. The majority of the clinical isolates exhibited a high-level of resistance to oxyiminocephalosporins and carbapenems, and possessed self-transferable blaKPC-2-carrying plasmids with sizes ranging from 20 to 120 kb.



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    Image Search Results


    Dendrogram of the PFGE- SmaI and PFGE- ApaI profiles of MRSA isolated from insulin-dependent diabetic individuals generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and their molecular characterization by SCC mec typing and MLST. a. Isolates showing > 80% similarity ( clusters A and B) after digestion with SmaI . b. Band pattern of strain 735 N obtained by digestion with ApaI . N, nasal mucosa; O, oropharyngeal mucosa; S, susceptible; R, resistant. * International clones used as controls

    Journal: Annals of Clinical Microbiology and Antimicrobials

    Article Title: Molecular characterization of methicillin-resistant Staphylococcus aureus among insulin-dependent diabetic individuals in Brazil

    doi: 10.1186/s12941-020-00401-y

    Figure Lengend Snippet: Dendrogram of the PFGE- SmaI and PFGE- ApaI profiles of MRSA isolated from insulin-dependent diabetic individuals generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and their molecular characterization by SCC mec typing and MLST. a. Isolates showing > 80% similarity ( clusters A and B) after digestion with SmaI . b. Band pattern of strain 735 N obtained by digestion with ApaI . N, nasal mucosa; O, oropharyngeal mucosa; S, susceptible; R, resistant. * International clones used as controls

    Article Snippet: Fig. 3 Dendrogram of the PFGE- ApaI profiles of MSSA isolates generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and sequence types obtained by MLST.

    Techniques: Isolation, Generated, Clone Assay

    Dendrogram of the PFGE- ApaI profiles of MSSA isolates generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and sequence types obtained by MLST. Clustering of isolates digested with ApaI that were analyzed by MLST. All isolates except for 735 N were susceptible to methicillin (MSSA). N, nasal mucosa; O, oropharyngeal mucosa; arc C, carbamate kinase; aro E, shikimate dehydrogenase; glp F, glycerol kinase; gmk , guanylate kinase; pta , phosphate acetyltransferase; tpi , triosephosphate isomerase; yqi L, acetyl coenzyme A; ST, sequence type. Isolate 76 N was identified as ST398 in a previous study from our group. Isolate 700O was sent to the curator of the MLST database ( https://pubmlst.org/ ) for identification of the new ST. This isolate was identified as ST 6133

    Journal: Annals of Clinical Microbiology and Antimicrobials

    Article Title: Molecular characterization of methicillin-resistant Staphylococcus aureus among insulin-dependent diabetic individuals in Brazil

    doi: 10.1186/s12941-020-00401-y

    Figure Lengend Snippet: Dendrogram of the PFGE- ApaI profiles of MSSA isolates generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and sequence types obtained by MLST. Clustering of isolates digested with ApaI that were analyzed by MLST. All isolates except for 735 N were susceptible to methicillin (MSSA). N, nasal mucosa; O, oropharyngeal mucosa; arc C, carbamate kinase; aro E, shikimate dehydrogenase; glp F, glycerol kinase; gmk , guanylate kinase; pta , phosphate acetyltransferase; tpi , triosephosphate isomerase; yqi L, acetyl coenzyme A; ST, sequence type. Isolate 76 N was identified as ST398 in a previous study from our group. Isolate 700O was sent to the curator of the MLST database ( https://pubmlst.org/ ) for identification of the new ST. This isolate was identified as ST 6133

    Article Snippet: Fig. 3 Dendrogram of the PFGE- ApaI profiles of MSSA isolates generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and sequence types obtained by MLST.

    Techniques: Generated, Sequencing